| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 1961 | BERNIER, Kaitlyn | UMass Dartmouth |
25.70 |
| 3 | 1994 | DOUGLAS, Jahela | Moore Elite Track Club | 25.50 |
| 4 | 1311 | MALLETTE, Christine | RPI |
25.41 |
| 5 | 201 | WYSE, Schyler | Wheaton (Mass.) |
25.41 |
| 6 | 1309 | MCGUINN, Emma | RPI |
25.70 |
| 7 | 1825 | HILTUNEN, Devin | Brandeis |
25.70 |