| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 1353 | HON, Maddy | MIT |
NT |
| 3 | 1335 | MILLER, JoJo | MIT |
12.87 |
| 4 | 295 | WOODEN, Wendy | Wheaton (Mass.) |
12.41 |
| 5 | 573 | ESCALERA, Jailene | Bridgewater St. |
12.18 |
| 6 | 743 | REDMOND, Sophia | Mary Washington |
12.21 |
| 7 | 248 | WICKENHEISSER, Kate | Wheaton (Mass.) |
12.77 |
| 8 | 1337 | LO, Abigail | MIT |
13.48 |