| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 1337 | LO, Abigail | MIT |
27.39 |
| 2 | 1353 | HON, Maddy | MIT |
26.60 |
| 3 | 694 | HAYES, Trinity | RIC |
25.46 |
| 4 | 1753 | HUGHES, Heloise | Amherst |
25.12 |
| 5 | 341 | KELEHER, Ashley | Colby-Sawyer |
24.97 |
| 6 | 573 | ESCALERA, Jailene | Bridgewater St. |
25.03 |
| 7 | 743 | REDMOND, Sophia | Mary Washington |
25.39 |
| 8 | 1335 | MILLER, JoJo | MIT |
25.82 |